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GeneChip^® Human Genome U133 Plus 2.0 Array

First and most comprehensive whole human genome expression array

Convenient one-array view.
Complete coverage of the Human Genome U133 Set plus 6,500 additional genes for analysis of over 47,000 transcripts.
Use the Power of the Probe Set and get multiple independent measurements for each transcript that deliver the greatest accuracy and reproducibility of any microarray platform.
GeneChip® System: Richer data, sharper insights, better decisions

Array Profile

All probe sets represented on the GeneChip Human Genome U133 Set are identically replicated on the GeneChip Human Genome U133 Plus 2.0 Array. The sequences from which these probe sets were derived were selected from GenBank®, dbEST, and RefSeq. The sequence clusters were created from the UniGene database (Build 133, April 20, 2001) and then refined by analysis and comparison with a number of other publicly available databases, including the Washington University EST trace repository and the University of California, Santa Cruz Golden-Path human genome database (April 2001 release).

In addition, there are 9,921 new probe sets representing approximately 6,500 new genes. These gene sequences were selected from GenBank, dbEST, and RefSeq. Sequence clusters were created from the UniGene database (Build 159, January 25, 2003) and refined by analysis and comparison with a number of other publicly available databases, including the Washington University EST trace repository and the NCBI human genome assembly (Build 31).

Data Sheets

Human Genome U133 Arrays (pdf, 1.76 MB)

Technical Notes

Design and Performance of the GeneChip® Human Genome U133 Plus 2.0 and Human Genome U133A 2.0 Array (pdf, 207 KB)

GeneChip® Expression Platform: Comparison, Evolution, and Performance (pdf, 269 KB)

Application Notes

Microarray Applications in Infectious Disease (pdf, 252 KB)

Microarrays in Cancer Research (pdf, 423 KB)

Part #	Description	Details
900470	GeneChip® Human Genome U133 Plus 2.0 Array	Contains 2 arrays
900466	GeneChip® Human Genome U133 Plus 2.0 Array	Contains 6 arrays
900467	GeneChip® Human Genome U133 Plus 2.0 Array	Contains 30 arrays

Array & Reagent Bundles

Part #	Description	Details
901226	GeneChip® Human Genome U133 Plus 2.0 Array and 3' IVT Express Kit Bundle	Sufficient for 10 reactions
901227	GeneChip® Human Genome U133 Plus 2.0 Array and 3' IVT Express Kit Bundle	Sufficient for 30 reactions

Required Products

Part #	Description	Details
00-0211	GeneChip Scanner 3000 7G with Workstation
	GeneChip® Operating Software	Includes the GeneChip Scanner 3000 High-Resolution Scanning Patch. See Product Page.
	* GeneChip Scanner 3000 High-Resolution Update is standard on all instruments shipped starting in September 2003 with serial number series 502. Previous versions, serial number series 501, will require the 00-0110 GeneChip Scanner 3000 High-Resolution Update to be installed.

Related Products

Part #	Description	Details
	Assays and Reagents	See Product Page
900686	GeneChip® HT One-Cycle Target Labeling and Control Kits	Sufficient for 96 reactions

FAQs

Gene Expression Arrays

Human Genome U133 2.0 Arrays

Manuals

3' IVT Express Kit User Manual (pdf, 3.47 MB)

Alignment Data in PSL Format

Expression Analysis Technical Manual, with Specific Protocols for Use with the Hybridization, Wash, and Stain Kit (pdf, 2.1 MB)

Probe Set Data in MAGE-ML Format

Array Comparisons

HG-U95 to HG-U133 Plus, Complex (zip, 37 MB)

Bovine to HG-U133 Plus, Best Match (zip, 100 KB)

Bovine to HG-U133 Plus, Complex (zip, 598 KB)

Bovine to HG-U133 Plus, Good Match (zip, 103 KB)

Bovine to HG-U133 Plus, No Match (zip, 27 KB)

Canine 2.0 to HG-U133 Plus, Best Match (zip, 164 KB)

Canine 2.0 to HG-U133 Plus, Complex Match (zip, 1.4 MB)

Canine 2.0 to HG-U133 Plus, Good Match (zip, 182 KB)

Canine 2.0 to HG-U133 Plus, No Match (zip, 32 KB)

Canine to HG-U133 Plus, All (zip, 433 KB)

Canine to HG-U133 Plus, Best Match (zip, 19 KB)

Canine to HG-U133 Plus, Good Match (zip, 20 KB)

Canine to HG-U133 Plus, No Match (zip, 30 KB)

HG-U133 Plus 2.0 to Rhesus Best Match

HG-U133 Plus 2.0 to Rhesus Complex

HG-U133 Plus 2.0 to Rhesus Good Match

HG-U133 Plus 2.0 to Rhesus No Match

HG-U133 Plus to Human Exon 1.0 ST, Best Match (zip, 899 KB)

HG-U133 Plus to Human Exon 1.0 ST, Complex Match (zip, 36 MB)

HG-U133 Plus to Human Exon 1.0 ST, Good Match (zip, 937 KB)

HG-U133 Plus to Human Exon 1.0 ST, No Match (zip, 1.5 KB)

HG-U133 Plus to Human Gene 1.0 ST, Best Match (zip, 651 KB)

HG-U133 Plus to Human Gene 1.0 ST, Complex Match (zip, 2.7 MB)

HG-U133 Plus to Human Gene 1.0 ST, Good Match (zip, 658 KB)

HG-U133 Plus to Human Gene 1.0 ST, No Match (zip, 38 KB)

HG-U133 to HG-U133 Plus, Best Match (zip, 5 KB)

HG-U133 to HG-U133 Plus, Complex (zip, 24 MB)

HG-U133 to HG-U133 Plus, Good Match (zip, 5 KB)

HG-U133 to HG-U133 Plus, No Match (zip, 1 KB)

HG-U95 to HG-U133 Plus, Best Match (zip, 35 KB)

HG-U95 to HG-U133 Plus, Good Match (zip, 37 KB)

HG-U95 to HG-U133 Plus, No Match (zip, 1 KB)

Porcine to HG-U133 Plus, Best Match (zip, 109 KB)

Porcine to HG-U133 Plus, Complex (zip, 596 KB)

Porcine to HG-U133 Plus, Good Match (zip, 112 KB)

Porcine to HG-U133 Plus, No Match (zip, 28 KB)

Rat 230 to HG-U133 Plus, Best Match (zip, 1 KB)

Rat 230 to HG-U133 Plus, Complex (zip, 225 KB)

Rat 230 to HG-U133 Plus, Good Match (zip, 1 KB)

Rat 230 to HG-U133 Plus, No Match (zip, 79 KB)

Library Files

Human Genome U133 Plus 2.0 Array (zip, 30 MB)

Package Inserts

Human Genome U133 Plus 2.0 Array (pdf, 130 KB)

Alignment, Annotation, and Sequence Files

Information about using Alignment, Annotation, and Sequence files

NetAffx Alignment Files

HG-U133_Plus_2 Alignments to Genome, PSL (7.4 MB, 9/28/06)

Current NetAffx Annotation Files

HG-U133_Plus_2 Annotations, CSV format (20 MB, 7/8/08)

HG-U133_Plus_2 Annotations, MAGE-ML XML Format (85 MB, 7/9/08)

HG-U133_Plus_2 BLASTP annotations, CSV Format (11 KB, 7/8/08)

HG-U133_Plus_2 Orthologs/Homologs, CSV Format (7.4 MB, 7/8/08)

Note: NetAffx annotation files are intended for use in automated analysis. Some are not compatible with all spreadsheet applications. Please use the NetAffx Analysis Center to limit download data to your probe sets of interest. Annotation CSV files for the exon arrays are split into a probe set-level annotation file and a transcript cluster-level annotation file; exon array CSV files are compatible with ExACT.