The GeneChip® 3' IVT Express Kit is the latest and most effective option for target
preparation on Affymetrix' industry-standard 3' expression arrays.

The kits feature:

• Low RNA input requirements - as little as 50 ng total RNA for a single round of amplification
• Streamlined workflow - no initial primer annealing or cDNA purification step, and a 1.25-hour second-strand cDNA synthesis time
• The option to decrease target labeling time to a single day with appropriate inputs of total RNA
• Fewer reagents needed for entire amplification and labeling process
• A simple protocol for ease of use, convenience and a high rate of success
• Magnetic bead purification for enhanced recovery
• A complete kit that includes poly-A RNA and hybridization controls

With enhanced performance, fewer required reagents and a simple protocol, target preparation has never been so easy.

Components of the GeneChip 3' IVT Express Kit include:

• All reagents needed to generate and label aRNA target
• Poly-A RNA controls and hybridization controls
• Magnetic bead purification for enhanced recovery
• Plastic consumables needed to run the assay

The GeneChip 3' IVT Express Kit is available or offered in a 10-reaction and 30-reaction size. The kit can be purchased alone or bundled with popular 3' arrays for additional savings:

• GeneChip® Human Genome U133 Plus 2.0 Arrays (10 and 30 packs)
• GeneChip® Mouse Genome 430 2.0 Arrays (10 and 30 packs)
• GeneChip® Rat Genome 230 2.0 Arrays (10 and 30 packs)

Additional products needed to run the complete Affymetrix solution include the GeneChip® Hybridization, Wash and Stain Kit.

About Poly-A controls
Poly-A controls (lys, phe, thr and dap) monitor the target labeling process from start to finish. They serve as sensitivity indicators of target prep and labeling efficiency. This kit contains four exogenous, pre-mixed, poly-adenylated prokaryotic controls that are spiked directly into RNA samples before target labeling. Their resultant signal intensities on GeneChip arrays serve as indicators of the labeling reaction efficiency.

The 20X Eukaryotic Hybridization Controls are comprised of a mixture of biotinylated and fragmented cRNA of bioB, bioC, bioD and cre in staggered concentrations, ready to be added directly to the hybridization cocktail. These controls facilitate monitoring of the hybridization process for troubleshooting. Control Oligo B2 is included to provide alignment signals for image analysis.

Affymetrix will continue to supply both One- and Two-Cycle Reagents for a period of one year to allow completion of current projects. Current One- and Two-Cycle Target Labeling and Control Reagents will be available until September 2009.

Data Sheets

3' IVT Express Kit (pdf, 278 KB)

Technical Notes

3' IVT Express and One-Cycle Comparison (pdf, 245 KB)

Part #	Description	Details
901228	GeneChip® 3' IVT Express Kit	Sufficient for 10 reactions
901229	GeneChip® 3' IVT Express Kit	Sufficient for 30 reactions

Part #	Description	Details
901226	GeneChip® Human Genome U133 Plus 2.0 Array and 3' IVT Express Kit Bundle	Sufficient for 10 reactions
901227	GeneChip® Human Genome U133 Plus 2.0 Array and 3' IVT Express Kit Bundle	Sufficient for 30 reactions
901230	GeneChip® Mouse Genome 430 2.0 Array and 3' IVT Express Kit Bundle	Sufficient for 10 reactions
901231	GeneChip® Mouse Genome 430 2.0 Array and 3' IVT Express Kit Bundle	Sufficient for 30 reactions
901232	GeneChip® Rat 230 2.0 Array and 3' IVT Express Kit Bundle	Sufficient for 10 reactions
901233	GeneChip® Rat Genome 230 2.0 Array and 3' IVT Express Kit Bundle	Sufficient for 30 reactions

Related Products

Part #	Description	Details
900720	GeneChip® Hybridization, Wash and Stain Kit	Sufficient for 30 reactions

FAQs

3' IVT Express Kit

Manuals

3' IVT Express Kit User Manual (pdf, 3.47 MB)

Expression Wash, Stain and Scan User Manual for Cartridge Arrays (pdf, 5.41 MB)

Quick Reference Cards

3' IVT Express Kit Assay (pdf, 631 KB)

Sample Data

3' IVT Express Kit (zip, 150 MB)

Material Safety Data Sheets

3' IVT Express Kit (zip, 1.3 MB)

Package Inserts

3' IVT Express Kit (pdf, 566 KB)

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