The kit is intended for use with methods that isolate RNA from unfractionated human whole blood only, such as the PAXgene™ Blood RNA System from PreAnalytiX. To learn more about the PAXgene™ Blood RNA System visit www.PreAnalytiX.com.

The Blood RNA Concentration Kit provides reagents to concentrate purified blood RNA to a volume suitable for target preparation. A set of control RNA samples used to verify the globin reduction process is available in the Globin-Reduction RNA Controls.

As part of the completely integrated GeneChip® brand reagent product line for expression analysis, the Blood RNA Concentration Kit offers:

Increased assay sensitivity when working with human whole blood RNA samples
Use of the Kit improves assay sensitivity by reducing the amount of labeled globin cRNA produced during target preparation using a mixture of peptide nucleic acid (PNA) oligonucleotides complementary to human globin message (not included in this kit).

Streamlined experimental workflow
The kit seamlessly integrates the globin-reduction procedure into the GeneChip Target Labeling Assay, requiring minimal additional hands-on time.

Novel Control RNAs for validating and monitoring the globin reduction process
A set of control RNAs are available in the Globin-Reduction RNA Controls kit for assessing the effectiveness of the globin-reduction process, providing additional information not available when only processing whole blood samples.

The Blood RNA Concentration Kit box contains the following reagents

Reagent	Quantity
RNA Concentration Spin Columns	30 columns
RNA Binding Buffer	45 ml
RNA Wash Buffer	11 ml
RNase-free Water	10 ml
Collection Tubes (2ml)	60 tubes
Collection Tubes (1.5ml)	30 tubes
Buffer BR5	5 ml

Globin-Reduction RNA Controls that provide verification of the globin reduction process are also available.

Please note: The kit does not contain the PNA oligonucleotides required for the globin reduction process. These must be ordered separately as described in the protocol included with the Blood RNA Concentration Kit.

Data Sheets

Standardized Assays and Reagents for GeneChip Expression Analysis (pdf, 3.02 MB)

Technical Notes

An Analysis of Blood Processing Methods to Prepare Samples for GeneChip® Expression Profiling (pdf, 295 KB)

Part #	Description	Details
900585	GeneChip® Blood RNA Concentration Kit	Sufficient for 30 samples Note: The Globin-Reduction oligomers must be ordered separately.

Related Products

Part #	Description	Details
900586	Globin-Reduction RNA Controls	Sufficient for 12 reactions
900431	GeneChip® One-Cycle cDNA Synthesis Kit	Sufficient for 30 reactions
901229	GeneChip® 3' IVT Express Kit	Sufficient for 30 reactions

FAQs

Blood Sample Processing

Gene Expression Assay and Data Analysis

Manuals

Globin-Reduction Protocol (pdf, 495 KB)

Probe Set Data in MAGE-ML Format

Material Safety Data Sheets

Blood RNA Concentration Kit (zip, 91 KB)

Package Inserts

Globin-Reduction RNA Controls (pdf, 76 KB)

Additional Support

Fluidics Scripts

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