News and Events
12/9/2008
Affymetrix Technology Powers Cincinnati Children's Hospital's Innovative Test for Jaundice
12/8/2008
Affymetrix Completes Acquisition of Panomics

Plant and Animal Genome XVII Conference
1/10/2009-1/14/2009
Achieving ROI in Personalized Medicine: Barriers, Incentives, and Pathways to Commercialization
1/27/2009-1/28/2009
Scientific Publications

The Affymetrix Targeted Genotyping Bovine 25K SNP Kit provides genotype analysis of approximately 25,000 SNPs. This kit contains 10,000 SNPs from the original Bovine Mapping 10K SNP Kit as well as 15,000 novel SNPs. The SNPs on the panel were validated across 15 Bos taurus and Bos indicus breeds and include several quantitative trait loci (QTLs) of interest to beef and dairy cattle breeders. The panel also includes SNPs from two African Sanga breeds.
The majority of the SNPs were discovered as part of the Bovine Sequencing Project and were validated at Affymetrix. In both kits, genotype data is generated in a single multiplexed assay using proven molecular inversion probe (MIP) technology in combination with Affymetrix universal tag arrays, and scanned on an Affymetrix GeneChip® Scanner 3000 TG System.
Literature
Khatkar M. S., et al. A Primary Assembly of a Bovine Haplotype Block Map Based on a 15,036-Single-Nucleotide Polymorphism Panel Genotyped in Holstein-Friesian Cattle. Genetics 176:763-772 (2007).
Additional Materials
| Part # | Description | Details |
|---|---|---|
| 900919 | GeneChip® Bovine Mapping 25K SNP Kit | Kit contains enough reagents to process a total of 24 assays (23 customer samples and one kit control) |
| 901191 | GeneChip® Universal 70K Tag Array 24 Pack | Each pack contains 24 arrays, enough to process one Bovine Mapping 25K SNP Kit |
Related Products
| Part # | Description | Details |
|---|---|---|
| 000600 | GeneChip® Bovine Mapping 25K SNP Service | For more information on pricing, minimum number of samples or a list of third-party service providers, please consult the Affymetrix Services Home Page. |
Assay Panel Files
Bovine Mapping 25K SNP Kit (zip, 537 KB)
Manuals
Targeted Genotyping Analysis (GTGS) User's Guide, includes TG protocol (pdf, 1.9 MB)
Targeted Genotyping System Site Preparation Guide (pdf, 892 KB)
Targeted Genotyping System User's Guide (pdf, 4.04 MB)
Quick Reference Cards
Targeted Genotyping Quick Reference Card, includes TG Protocol (zip, 1.62 MB)
User Bulletin, Affymetrix Targeted Genotyping System (pdf, 69 KB)
Material Safety Data Sheets
Targeted Genotyping Wash Solution A (pdf, 26 KB)
Targeted Genotyping Wash Solution B (pdf, 26 KB)
Additional Support
Custom Panel Design Guide (zip, 143KB)
Custom Panel Design Request Form (zip, 12 KB)



