This kit provides both positive and negative control RNA samples to validate the globin-reduction procedure in conjunction with the GeneChip® Blood RNA Concentration Kit (P/N 900585).

The Globin-Reduction RNA Controls box contains 2 vials of RNA:

Reagent	Description
Jurkat RNA	Purified from Jurkat cells that contain negligible amounts of globin mRNAs, this control represents RNA purified from the leukocyte fraction of whole blood.
Jurkat + Globin RNA	This control RNA is the Jurkat RNA described above but spiked with in vitro-synthesized, polyadenylated globin transcripts. The Jurkat + Globin RNA control mimics RNA purified from unfractionated whole blood.

The kit provides sufficient Jurkat RNA and Jurkat + Globin RNA for six reactions each, for a total of twelve reactions.

Recommended globin-reduction controls and their expected results:

Sample	Rx	Globin Reduction	Total RNA Input (µg)	Replicates	Expected Result
Jurkat RNA	1	No	2	3	Optimal GeneChip array results are expected. This reaction is used as a reference to determine success of the GeneChip Target Labeling.
	2	Yes	2	3	Optimal GeneChip array results are expected. This reaction is used to verify that the globin reduction procedure does not affect non-globin transcripts.
Jurkat RNA + Globin RNA	3	No	2	3	Sub-optimal GeneChip results are expected due to a loss in assay sensitivity. This reaction is used to demonstrate the effects of high amounts of globin mRNAs on array analysis.
	4	Yes	2	3	Optimal GeneChip array results similar to those obtained using the Jurkat RNA control are expected. This reaction is used to demonstrate the increase in assay sensitivity due to the globin reduction procedure.

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Data Sheets

Standardized Assays and Reagents for GeneChip Expression Analysis (pdf, 3.02 MB)

Technical Notes

An Analysis of Blood Processing Methods to Prepare Samples for GeneChip® Expression Profiling (pdf, 295 KB)

Part #	Description	Details
900586	GeneChip® Globin-Reduction RNA Controls	Sufficient for 12 reactions

Related Products

Part #	Description	Details
900585	GeneChip® Blood RNA Concentration Kit	Sufficient for 30 samples
900431	GeneChip® One-Cycle cDNA Synthesis Kit	Sufficient for 30 reactions
901229	GeneChip® 3' IVT Express Kit	Sufficient for 30 reactions

FAQs

Blood Sample Processing

Gene Expression Assay and Data Analysis

Manuals

Globin-Reduction Protocol (pdf, 495 KB)

Probe Set Data in MAGE-ML Format

Material Safety Data Sheets

Globin-Reduction RNA Controls (zip, 30 KB)

Package Inserts

Globin-Reduction RNA Controls (pdf, 76 KB)

Additional Support

Fluidics Scripts

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