The GeneChip® HT One-Cycle cDNA Synthesis Kit (Manufactured by Invitrogen for Affymetrix) is uniquely configured and tested for GeneChip target labeling using the GeneChip® Array Station. It provides all necessary reagents in one convenient kit for synthesis of double-stranded cDNA containing the T7 promoter sequence from total RNA.

The resultant cDNA is then purified and serves as a template in the subsequent in vitro transcription (IVT) reaction for complementary RNA (cRNA) amplification and biotin labeling to hybridize to GeneChip® brand arrays.

As part of the completely integrated GeneChip reagent product line for expression analysis using the GeneChip Array Station, the GeneChip HT One-Cycle cDNA Synthesis Kit offers:

• Streamlined workflow and 96-reaction packaging, well suited for large-scale studies using the GeneChip Array Station
• Optimal results based on a proven, robust protocol
• Consistent performance with SuperScript™ II from Invitrogen for reverse transcription
• Limited license to Van Gelder/Eberwine patents via Incyte

Component Name	Quantity	Volume
T7-Oligo(dT) Primer, 50 µM	1 tube	130 µL
5X 1st Strand Reaction Mix	1 tube	460 µL
DTT, 0.1M	1 tube	230 µL
dNTP, 10 mM	1 tube	460 µL
SuperScript™ II	1 tube	120 µL
5X 2nd Strand Reaction Mix	1 tube	3,300 µL
E.coli DNA Ligase	1 tube	110 µL
E.coli DNA Polymerase I	1 tube	440 µL
RNase H	1 tube	110 µL
T4 DNA Polymerase	1 tube	280µL
5X T4 DNA Polymerase Buffer	1 tube	60 µL

The HT One-Cycle cDNA Synthesis Kit is also available as part of the complete GeneChip® HT One-Cycle Target Labeling and Controls Kit.

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Data Sheets

Standardized Assays and Reagents for GeneChip Expression Analysis (pdf, 3.02 MB)

Part #	Description	Details
900687	GeneChip® HT One-Cycle cDNA Synthesis Kit (Can also be ordered as part of the HT One-Cycle Target Labeling and Controls Kit)	Sufficient for 96 reactions

Related Products

Part #	Description	Details
900686	GeneChip® HT One-Cycle Target Labeling and Controls Kit	Sufficient for 96 reactions
900688	GeneChip® HT IVT Labeling Kit	Sufficient for 96 reactions
00-0162 00-0235	GeneChip® Array Station	See Product Page

FAQs

Gene Expression Assay and Data Analysis

Manuals

Expression Analysis Technical Manual - Cartridge Arrays Using the Array Station (pdf, 4.5 MB)

Expression Analysis Technical Manual For HT Array Plates Using the GeneChip® Array Station (pdf, 7.4 MB)

Probe Set Data in MAGE-ML Format

Material Safety Data Sheets

HT One-Cycle cDNA Synthesis Kit (zip, 204 KB)

Package Inserts

GeneChip® HT IVT Labeling Kit (pdf, 129 KB)

HT One-Cycle cDNA Synthesis Kit (pdf, 125 KB)

Additional Support

Fluidics Scripts

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