The Affymetrix GeneChip® Mapping 100K Assay, in conjunction with the GeneChip® Mapping 100K Set, is designed to detect greater than 100,000 Single Nucleotide Polymorphisms (SNPs) in samples of genomic DNA.

The Mapping 100K Set is comprised of two arrays and two assay kits. Each array and its corresponding assay kit are processed independently of the other array and assay kit. The protocol starts with 250 ng of genomic DNA and utilizes a strategy that reduces the complexity of human genomic DNA up to 10 fold by first digesting the genomic DNA with the Xba I or Hind III restriction enzyme and then ligating sequences onto the DNA fragments. The complexity is further reduced by a PCR procedure optimized for fragments of a specified size range. Following these steps the PCR products (amplicons) are fragmented, end-labeled, and hybridized to a GeneChip array.

Data Sheets

Genome-Wide Human SNP Array 5.0 (pdf, 589 KB)

Application Notes

Microarrays in Cancer Research (pdf, 423 KB)

Part #	Description	Details
900520	GeneChip® Mapping 50K Xba Assay Kit	Sufficient for 30 reactions
900521	GeneChip® Mapping 50K Hind Assay Kit	Sufficient for 30 reactions
900807	GeneChip® Human Mapping 50K Xba Adaptor	Sufficient for 30 reactions
900808	GeneChip® Human Mapping 50K Hind Adaptor	Sufficient for 30 reactions
900534	PCR Primer 001	Sufficient for 100 PCR reactions

Required Products

Part #	Description	Details
	Affymetrix® GeneChip® Command Console® Software	Available Via Download
	Affymetrix® Genotyping Console™ Software	Available Via Download
00-0079	GeneChip® Fluidics Station 450
800138	GeneChip® Hybridization Oven 640
00-0211	GeneChip® Scanner 3000 7G

Related Products

Part #	Description	Details
901153	Genome-Wide Human SNP Array 6.0
901167	Genome-Wide Human SNP Array 5.0

Manuals

100K Mapping Assay Manual (pdf, 1.2 MB)

Probe Set Data in MAGE-ML Format

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