News and Events
12/9/2008
Affymetrix Technology Powers Cincinnati Children's Hospital's Innovative Test for Jaundice
12/8/2008
Affymetrix Completes Acquisition of Panomics
Plant and Animal Genome XVII Conference
1/10/2009-1/14/2009
Achieving ROI in Personalized Medicine: Barriers, Incentives, and Pathways to Commercialization
1/27/2009-1/28/2009
Scientific Publications
GeneChip® Mapping 500K Assay
Figure 1
The Affymetrix GeneChip® Mapping 500K Assay, in conjunction with the GeneChip® Mapping 500K Set, is designed to detect greater than 500,000 Single Nucleotide Polymorphisms (SNPs) in samples of genomic DNA. The Mapping 500K Set is comprised of two arrays and two assay kits. Each array and its corresponding assay kit are processed independently of the other array and assay kit. The protocol starts with 250 ng of genomic DNA per array and will generate SNP genotype calls for approximately 250,000 SNPs for each array of the two array set. The assay utilizes a strategy that reduces the complexity of human genomic DNA up to 10 fold by first digesting the genomic DNA with the Nsp I or Sty I restriction enzyme and then ligating sequences onto the DNA fragments. The complexity is further reduced by a PCR procedure optimized for fragments of a specified size range. Following these steps the PCR products (amplicons) are fragmented, end-labeled, and hybridized to a GeneChip array.
For more information, please review the GeneChip® Mapping 500K Assay Manual (pdf, 3.4 MB)
| Part # | Description | Details |
|---|---|---|
| 900766 | GeneChip® Mapping 250K Nsp Assay Kit | Sufficient for 30 reactions |
| 900753 | GeneChip® Mapping 250K Nsp Assay Kit | Sufficient for 100 reactions |
| 900765 | GeneChip® Mapping 250K Sty Assay Kit | Sufficient for 30 reactions |
| 900754 | GeneChip® Mapping 250K Sty Assay Kit | Sufficient for 100 reactions |
| 900804 | GeneChip® Human Mapping 250K Nsp Adaptor | Sufficient for 100 reactions |
| 900805 | GeneChip® Human Mapping 250K Sty Adaptor | Sufficient for 100 reactions |
| 900806 | GeneChip® Human Mapping 250K PCR Primer 002 | Sufficient for 150 reactions |
Required Products
| Part # | Description | Details |
|---|---|---|
| Affymetrix® GeneChip® Command Console® Software | Available Via Download | |
| Affymetrix® Genotyping Console™ Software | Available Via Download | |
| 00-0079 | GeneChip® Fluidics Station 400 or Fluidics Station 450/250 | |
| 800138 | GeneChip® Hybridization Oven | |
| 00-0186 | GeneChip® Scanner 3000 with high-resolution scanning patch |
Related Products
| Part # | Description | Details |
|---|---|---|
| 901153 | Genome-Wide Human SNP Array 6.0 | |
| 901167 | Genome-Wide Human SNP Array 5.0 |