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Affymetrix will continue to supply both One- and Two-Cycle Reagents for a period of one year to allow completion of current projects. Current One- and Two-Cycle Target Labeling and Control Reagents will be available until September 2009.

The One-Cycle Target Labeling and Control Reagent package provides all necessary labeling and control reagents with one convenient part number for target preparation from 1 - 15 µg of total RNA or 0.2 - 2 µg of mRNA.

Functionally tested and validated on GeneChip arrays, these reagents provide optimal performance and ensure robust results.

Component Name	Quantity
GeneChip® IVT Labeling Kit	1
GeneChip® One-Cycle cDNA Synthesis Kit	1
GeneChip® Sample Cleanup Module	1
GeneChip® Poly-A RNA Control Kit	1
GeneChip® Hybridization Control Kit	1

Individual component kits can also be ordered separately.

Data Sheets

Standardized Assays and Reagents for GeneChip Expression Analysis (pdf, 3.02 MB)

Part #	Description	Details
900493	GeneChip® One-Cycle Target Labeling and Control Reagents	Sufficient for 30 reactions

Related Products

Part #	Description	Details
900449	GeneChip® IVT Labeling Kit	Sufficient for 30 reactions
900431	GeneChip® One-Cycle cDNA Synthesis Kits	Sufficient for 30 reactions
900494	GeneChip® Two-Cycle Target Labeling and Control Reagents	Sufficient for 30 reactions
901229	GeneChip® 3' IVT Express Kit	Sufficient for 30 reactions
900375	GeneChip® T7-Oligo(dT) Promoter Primer Kit	Sufficient for 150 reactions
900301	Control Oligo B2, 3nM	Sufficient for 30 samples

FAQs

Gene Expression Assay and Data Analysis

Manuals

Expression Analysis Technical Manual, with Specific Protocols for Use with the Hybridization, Wash, and Stain Kit (pdf, 2.1 MB)

Probe Set Data in MAGE-ML Format

Additional Support

Fluidics Scripts

Presentation, GeneChip® 3'- Amplification Reagents: One-Cycle Target Labeling Assay and One-Cycle cDNA Synthesis Kit (pdf, 1 MB)

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