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These Affymetrix® Mapping 500K Assay tutorials describe how to run the 500K assay protocol to achieve higher call rates. This presentation provides a detailed overview of the steps included in the 500K manual, and covers key topics such as equipment and calibration, reagents, consumables, sample processing, operational management, workflow, ramp-up and support.

Mapping 500K Assay Tutorials

The accompanying videos contain information on key stages of the 500K assay, including: Overview, Equipment/Calibration, Reagents, Consumables, Sample Processing, Operational Management, Workflow, Ramp-Up and Support.

Introduction - (15:14)

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Stage 1: DNA Preparation (2:48)

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Includes: Detailed Description

Stage 2: Digestion (7:37)

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Includes: Detailed Description

Stage 3: Ligation (6:22)

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Stage 4: PCR (10:46)

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Stage 5: Purification and Elution (9:33)

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Stage 6: Quantitation/Normalization (10:56)

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Stage 7: Fragmentation (8:53)

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Stage 8: Labeling (4:47)

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Stage 9: Hybridization (13:23)

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Stage 10: Quality Control (6:23)

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Stage 11: Review (2:17)

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Related Content

• Genotyping Console™ Software
• The Affymetrix Mapping 500K Array Set
• Affymetrix Microarray Bulletin Association Articles

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