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Whole-transcript (WT) Sense Target Assay for use with GeneChip® Exon and Gene Array Designs

The Affymetrix Whole-transcript Sense Target Labeling Assay is designed to generate unbiased, amplified and biotinylated sense-strand DNA targets from the entire expressed genome. Using the Whole-transcript Assay in combination with GeneChip® Exon 1.0 ST and Gene 1.0 ST Arrays allows you to measure expression across the entire length of a gene, thus offering a more complete and accurate picture of overall gene expression than 3'-based assays.

Whole-transcript (WT) Sense Target Assay Tutorial

The training covers the following sections:

• Exon and Gene-level Array Designs
• Advantages of the WT Assay
• WT Assay Workflow
• Ribosomal RNA Removal for Exon Arrays
• WT Assay Protocol Steps
• Assay Quality Metrics and Performance

View the WT Assay Tutorial (32:57)

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Related Content

• Genechip Exon and Gene Array System
• Whole Transcript (WT) Sense Target Labeling Manual (pdf, 795KB)
• Whole Transcript (WT) Expression Analysis Application Note (pdf, 562KB)